ثريد | مبادئ التفاعلات الكيميائية في شريط فحص البول
Thread | Principles of chemical reactive in urine-strip
Thread | Principles of chemical reactive in urine-strip
الثريد هذا موجة لجميع طلبة المختبرات والمتدربين والعاملين في قسم urine analysis
1- Blood
Principle of procedure: based on the Pseudo-peroxidase activity of the haem moiety of hemoglobin and myoglobin. The chromogen is oxidized by a hydroperoxide in the presence of haem and changes color from yellow (or greenish yellow) to blue.
Principle of procedure: based on the Pseudo-peroxidase activity of the haem moiety of hemoglobin and myoglobin. The chromogen is oxidized by a hydroperoxide in the presence of haem and changes color from yellow (or greenish yellow) to blue.
2- Bilirubin
Principle of procedure: Azo-coupling reaction of bilirubin with a diazonium salt in an acid medium to form an azodye. Color changes from light tan to beige or light pink.
Principle of procedure: Azo-coupling reaction of bilirubin with a diazonium salt in an acid medium to form an azodye. Color changes from light tan to beige or light pink.
3- Urobilinogen
Principle of procedure: The test is based on the Ehrlich's reaction. Color changes from light orange-pink to dark pink.
Principle of procedure: The test is based on the Ehrlich's reaction. Color changes from light orange-pink to dark pink.
4- ketones
based on the reaction of acetoacetic acid in urine with nitroprusside. The resulting color ranges from tan, when no reaction takes place, to different purple shades for positive reactions.
based on the reaction of acetoacetic acid in urine with nitroprusside. The resulting color ranges from tan, when no reaction takes place, to different purple shades for positive reactions.
5- Glucose
Principle of procedure : Glucose oxidase catalyzes the oxidation of glucose to form hydroger peroxide. The hydrogen peroxide thus formed then oxidizes a chromogen on the reaction pad by the action of peroxidase.
Principle of procedure : Glucose oxidase catalyzes the oxidation of glucose to form hydroger peroxide. The hydrogen peroxide thus formed then oxidizes a chromogen on the reaction pad by the action of peroxidase.
6- Proteins
based on the color change of the indicator, tetra- bromophenol blue, in the presence of proteins. A positive reaction is indicated by a color change from greenish-yellow to green and then to dark-green.
based on the color change of the indicator, tetra- bromophenol blue, in the presence of proteins. A positive reaction is indicated by a color change from greenish-yellow to green and then to dark-green.
7-Nitrite
The test is based on the diazotization reaction of nitrite with an aromatic amine to produce a diazonium salt. It is followed by an azo-coupling reaction of this diazonium salt with an aromatic compound on the reaction pad. That causes a color change form white to pink.
The test is based on the diazotization reaction of nitrite with an aromatic amine to produce a diazonium salt. It is followed by an azo-coupling reaction of this diazonium salt with an aromatic compound on the reaction pad. That causes a color change form white to pink.
8-Leukocyte
Reveals the presence of granulocyte esterases. The esterases cleave a derivatized pyrazol ester to release derivatized hydroxypyrazol. This reacts with diazonium salt to produce a purple product.
Reveals the presence of granulocyte esterases. The esterases cleave a derivatized pyrazol ester to release derivatized hydroxypyrazol. This reacts with diazonium salt to produce a purple product.
9- PH
Principle of procedure : Double indicator system. Indicator's methyl red and bromothymol blue are used to give distinct color changes from orange to green to blue.
Principle of procedure : Double indicator system. Indicator's methyl red and bromothymol blue are used to give distinct color changes from orange to green to blue.
10-Specific Gravity (SG)
Principle of procedure : lonic solutes present in the urine cause protons to be released from a polyelectrolyte. As the protons are released the pH decreases and produces a color change of bromothymol blue from blue-green to yellow-green.
Principle of procedure : lonic solutes present in the urine cause protons to be released from a polyelectrolyte. As the protons are released the pH decreases and produces a color change of bromothymol blue from blue-green to yellow-green.
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